User manual
CRISPRko-Ready ioGlutamatergic Neurons
cat no io1090S | Human iPSC-derived glutamatergic neurons expressing Cas9 for rapid gene knockout generation
cat no io1090S | Human iPSC-derived glutamatergic neurons expressing Cas9 for rapid gene knockout generation
CRISPRko-Ready ioGlutamatergic Neurons mature rapidly into functional glutamatergic neurons after revival in the recommended medium. These cells stably express Cas9 nuclease so users can perform single gene knockouts, pooled CRISPR screens or arrayed CRISPR screens. CRISPRko-Ready ioGlutamatergic Neurons are delivered in a convenient cryopreserved format and provide a homogeneous and reproducible model of human excitatory neurons.
The user manual provides full details for cell culture and guide RNA delivery by either lentivirus transduction or lipid-based transfection and includes:
- Culture of CRISPRko-Ready ioGlutamatergic Neurons
- Lipid based delivery of synthetic guide RNA, including a protocol optimised for 96-well plate format
- Lentiviral delivery of guide RNA
- Immunofluorescence readout
DOC-3012 2.0
51±¬ÁÏ
2025